Monkey IL-8 ELISA Kit (ab119550)
Key features and details
- Sensitivity: 3 U/ml
- Range: 19.5 U/ml - 1250 U/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
Overview
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Product name
Monkey IL-8 ELISA Kit
See all IL-8 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 6.3% Inter-assay Sample n Mean SD CV% Overall 8.7% -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
3 U/ml -
Range
19.5 U/ml - 1250 U/ml -
Recovery
88 %
Sample specific recovery Sample type Average % Range Serum 88 72% - 125% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Monkey -
Product overview
Abcam’s IL-8 Monkey ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of monkey IL-8 in culture supernatant and serum (cynomolgus, baboon).
IL-8 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a biotin-conjugated anti-monkey IL-8 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-8 captured on the plate.
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Notes
Standard is recombinant protein produced in E.coli.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-monkey IL8 polyclonal antibody 1 x 70µl Microplate coated with monoclonal antibody to monkey IL-8 (12 x 8 wells) 1 unit Monkey IL-8 Standard Concentrate (125,000 U/mL) 2 x 100µl Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
Research areas
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Function
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- (Ala-IL-8)77
- (Ser-IL-8)72
- 9E3
see all
Images
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Typical Standard Curve
Representative Standard Curve using ab119550.