Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21857] to LYVE1 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-LYVE1 antibody [EPR21857] - BSA and Azide free
    See all LYVE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21857] to LYVE1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon tissue.

  • General notes

    Ab232935 is the carrier-free version of ab219556. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232935 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on blood sinusoids of human liver (PMID: 11719431) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219556).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphatic endothelial cells of human tonsil (PMID: 11377291) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219556).

  • Immunoprecipitation - Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)
    Immunoprecipitation - Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)

    LYVE1 was immunoprecipitated from 0.35 mg of human fetal lung lysate with ab219556 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab219556 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human fetal lung lysate 10 μg (Input).
    Lane 2: ab219556 IP in human fetal lung lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219556 in human fetal lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 20 seconds.

    The band observed is consistent with the soluble LYVE1 described in the literature (PMID: 29262593; PMID: 26966180; PMID: 26926389).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219556).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphatic endothelial cells of human colon (PMID: 11377291) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219556).

  • Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)
    Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-LYVE1 antibody [EPR21857] - BSA and Azide free (ab232935)

© 2021 Astana Biomed Group LLP. All rights reserved.