Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 117 264 ₸

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Anti-LYVE1 antibody [EPR21857] (ab219556)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21857] to LYVE1
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-LYVE1 antibody [EPR21857]
See all LYVE1 primary antibodies
Description

Rabbit monoclonal [EPR21857] to LYVE1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human lymph node, brain, fetal lung and fetal spleen lysates. IHC-P: Human colon, tonsil and liver tissue. IP: Human fetal lung lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21857
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphatic endothelial cells of human tonsil (PMID: 11377291) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphatic endothelial cells of human colon (PMID: 11377291) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-LYVE1 antibody [EPR21857] (ab219556)

All lanes : Anti-LYVE1 antibody [EPR21857] (ab219556) at 1/1000 dilution

Lane 1 : Human fetal lung lysate
Lane 2 : Human lymph node lysate
Lane 3 : Human fetal spleen lysate
Lane 4 : Human brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 35 kDa
Observed band size: 50-70 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST

Exposure times:

Lane 1: 37 seconds

Lanes 2 & 4: 92 seconds

Lane 3: 6 seconds

Negative control: Human brain (PMID: 20599941).

Bands observed are consistent with full-length and soluble fragments, as described in the literature (PMID: 29262593, PMID: 26966180, PMID: 26926389).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on blood sinusoids of human liver (PMID: 11719431) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-LYVE1 antibody [EPR21857] (ab219556)

LYVE1 was immunoprecipitated from 0.35 mg of human fetal lung lysate with ab219556 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab219556 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: Human fetal lung lysate 10 μg (Input).
Lane 2: ab219556 IP in human fetal lung lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219556 in human fetal lung lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 20 seconds.

The band observed is consistent with the soluble LYVE1 described in the literature (PMID: 29262593; PMID: 26966180; PMID: 26926389).
Anti-LYVE1 antibody [EPR21857] (ab219556)

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