Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LYVE1 antibody [EPR21857] (ab219556)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21857] to LYVE1
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-LYVE1 antibody [EPR21857]
    See all LYVE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21857] to LYVE1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human lymph node, brain, fetal lung and fetal spleen lysates. IHC-P: Human colon, tonsil and liver tissue. IP: Human fetal lung lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphatic endothelial cells of human tonsil (PMID: 11377291) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphatic endothelial cells of human colon (PMID: 11377291) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-LYVE1 antibody [EPR21857] (ab219556)
    Western blot - Anti-LYVE1 antibody [EPR21857] (ab219556)
    All lanes : Anti-LYVE1 antibody [EPR21857] (ab219556) at 1/1000 dilution

    Lane 1 : Human fetal lung lysate
    Lane 2 : Human lymph node lysate
    Lane 3 : Human fetal spleen lysate
    Lane 4 : Human brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 50-70 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST

    Exposure times: 

    Lane 1: 37 seconds

    Lanes 2 & 4: 92 seconds

    Lane 3: 6 seconds

    Negative control: Human brain (PMID: 20599941).

    Bands observed are consistent with full-length and soluble fragments, as described in the literature (PMID: 29262593, PMID: 26966180, PMID: 26926389).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on blood sinusoids of human liver (PMID: 11719431) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-LYVE1 antibody [EPR21857] (ab219556)
    Immunoprecipitation - Anti-LYVE1 antibody [EPR21857] (ab219556)

    LYVE1 was immunoprecipitated from 0.35 mg of human fetal lung lysate with ab219556 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab219556 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human fetal lung lysate 10 μg (Input).
    Lane 2: ab219556 IP in human fetal lung lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219556 in human fetal lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 20 seconds.

    The band observed is consistent with the soluble LYVE1 described in the literature (PMID: 29262593; PMID: 26966180; PMID: 26926389).

  • Anti-LYVE1 antibody [EPR21857] (ab219556)
    Anti-LYVE1 antibody [EPR21857] (ab219556)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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