Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LYVE1 antibody [EPR21771] (ab218535)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21771] to LYVE1
  • Suitable for: IP, WB, IHC-P, IHC-Fr, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-LYVE1 antibody [EPR21771]
    See all LYVE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21771] to LYVE1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lymph node and lung lysates; bEnd.3 whole cell lysate. IHC-P: Mouse liver, lung and colon tissues. IHC-Fr: Mouse liver and stomach tissues. Flow Cyt: bEnd.3 cells. IP: Mouse lung lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21771

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling LYVE1 with ab218535 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the lymphatic endothelial cells of mouse lung is observed. Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
    Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling LYVE1 with ab218535 at 1/5000 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining of the endothelium of lymph vessels in the submucosae on mouse stomach tissue section (PMID: 15705793).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling LYVE1 with ab218535 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the lymphatic endothelium of mouse colon (PMID: 14722766). Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Western blot - Anti-LYVE1 antibody [EPR21771] (ab218535)
    Western blot - Anti-LYVE1 antibody [EPR21771] (ab218535)
    All lanes : Anti-LYVE1 antibody [EPR21771] (ab218535) at 1/1000 dilution

    Lane 1 : Mouse lymph node lysate
    Lane 2 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate
    Lane 3 : Mouse lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 34-70 kDa
    why is the actual band size different from the predicted?



     

    Exposure time : Lane 1: 3 minutes; Lane 2: 32 seconds; Lane 3: 5 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Several bands are observed including soluble, glycosylated and non-glycosylated forms which are consistent with the literature (PMID: 26966180).

  • Immunoprecipitation - Anti-LYVE1 antibody [EPR21771] (ab218535)
    Immunoprecipitation - Anti-LYVE1 antibody [EPR21771] (ab218535)

    LYVE1 was immunoprecipitated from 0.35 mg mouse lung lysate with ab218535 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218535 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Mouse lung lysate 10 µg (Input).

    Lane 2: ab218535 IP in mouse lung lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218535 in mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    Several bands are observed including soluble, glycosylated and non-glycosylated forms which are consistent with the literature (PMID: 26966180).

  • Flow Cytometry - Anti-LYVE1 antibody [EPR21771] (ab218535)
    Flow Cytometry - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Flow cytometric analysis of bEnd.3 (mouse brain endothelioma cell line) cells labeling LYVE1 with ab218535 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on total viable cells.

  • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
    Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling LYVE1 with ab218535 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining of the endothelium of sinusoid blood vessels on mouse liver tissue section (PMID: 11719431).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling LYVE1 with ab218535 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the endothelial surface of mouse hepatic sinusoids (PMID: 16353487; PMID: 11719431). Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Anti-LYVE1 antibody [EPR21771] (ab218535)
    Anti-LYVE1 antibody [EPR21771] (ab218535)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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