Immunohistochemical analysis of paraffin-embedded mouse intestine tissue staining LYVE-1 with ab14917. Positive staining is shown in the lymphatic endothelial cells (red).

Immunocytochemistry/immunofluorecent analysis of mouse colon tissue labelling LYVE-1 with ab14917 (red).

At the time point of 75dpi spirochetes (Bb) were not observed in association with the lymphatic-like vessels (LYVE-1) that run parallel to the sagittal sinus (SS, arrow) of the dura mater.

Dura samples were collected from transcardially perfused mice by craniotomy and post-fixed in 4% paraformaldehyde for 24h at 4°C. Samples were permeabilized in 0.1% Triton X-100, washed 3 times, and serum-blocked in 2.5% goat serum/PBS containing 1:100 dilution of Fc block. For B. burgdorferi staining, each sample was incubated in 1:100 dilution of rat anti-mouse unconjugated monoclonal anti-CD31 IgG, and 1:50 dilution biotinylated rabbit anti-B. burgdorferi polyclonal IgG at 4°C overnight. On the following day, the samples were washed, and stained with 1:100 dilution of Alexa 555 goat anti-rat polyclonal IgG, and 1:200 dilution of Alexa 488 streptavidin{"type":"entrez-protein","attrs":{"text":"S11223","term_id":"112468","term_text":"pir||S11223"}} for 1 hour at room temperature, covered from light. Secondary antibody-only controls for B. burgdorferi indirect fluorescent assay were performed in vitro and no fluorescence was observed.

Some of the dura samples were also stained for lymphatic vessels in a separate step, using 1:200 ab14917, followed by washing and secondary staining with 1:200 Alexa 633 goat-anti rabbit polyclonal IgG (yellow).

ab14917 at a 1/100 dilution staining LYVE1 from mouse tuberculosis infected lung by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes and then detected with an HRP conjugated goat anti-rabbit antibody.

 

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ab14917 staining LYVE1 in mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a EDTA-buffer pH 9.0. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 20°C. A biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

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ab14917 at 1/2000 dilution staining Ha-Ras transgenic mouse bladder (cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the primary antibody for 12 hours. A biotinylated polyclonal antibody was used as the secondary.

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