Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFL7 antibody [EPR22603-113] (ab256451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22603-113] to EGFL7
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-EGFL7 antibody [EPR22603-113]
    See all EGFL7 primary antibodies

  • Description

    Rabbit monoclonal [EPR22603-113] to EGFL7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human placenta lysate. HUVEC whole cell lysate. IHC-P: Human pancreas and placenta tissue. ICC/IF: HUVEC cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-EGFL7 antibody [EPR22603-113] (ab256451)
    Western blot - Anti-EGFL7 antibody [EPR22603-113] (ab256451)
    All lanes : Anti-EGFL7 antibody [EPR22603-113] (ab256451) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
    Lane 3 : Jurkat (human t cell leukemia t lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID: 18497746).

    Negative control: Jurkat (PMID: 15162510).

    Exposure time: 3 minutes.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFL7 antibody [EPR22603-113] (ab256451)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFL7 antibody [EPR22603-113] (ab256451)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (Human umbilical vein endothelial cell) cells labeling EGFL7 with ab256451 at 1/50, (10 µg/ml), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HUVEC cells. Negative control: Jurkat (PMID: 15162510). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFL7 antibody [EPR22603-113] (ab256451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFL7 antibody [EPR22603-113] (ab256451)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling EGFL7 with ab256451 at 1/500 dilution (1.12 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta (PMID: 24751645) is observed. The section was incubated with ab256451 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFL7 antibody [EPR22603-113] (ab256451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFL7 antibody [EPR22603-113] (ab256451)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling EGFL7 with ab256451 at 1/500 dilution (1.12 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels in human pancreas (PMID: 18497746) is observed. The section was incubated with ab256451 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Anti-EGFL7 antibody [EPR22603-113] (ab256451)
    Anti-EGFL7 antibody [EPR22603-113] (ab256451)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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