Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21771] to LYVE1 - BSA and Azide free
  • Suitable for: IHC-P, WB, IHC-Fr, IP, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-LYVE1 antibody [EPR21771] - BSA and Azide free
    See all LYVE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21771] to LYVE1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IHC-Fr, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lymph node and lung lysates; bEnd.3 whole cell lysate. IHC-P: Mouse liver, lung and colon tissues. IHC-Fr: Mouse liver and stomach tissues. Flow Cyt: bEnd.3 cells. IP: Mouse lung lysate.

  • General notes

    Ab230377 is the carrier-free version of ab218535. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230377 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling LYVE1 with ab218535 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the lymphatic endothelial cells of mouse lung is observed. Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218535).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling LYVE1 with ab218535 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the endothelial surface of mouse hepatic sinusoids (PMID: 16353487; PMID: 11719431). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218535).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)
    Immunoprecipitation - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)

    LYVE1 was immunoprecipitated from 0.35 mg mouse lung lysate with ab218535 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218535 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Mouse lung lysate 10 µg (Input).

    Lane 2: ab218535 IP in mouse lung lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218535 in mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    Several bands are observed including soluble, glycosylated and non-glycosylated forms which are consistent with the literature (PMID: 26966180).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218535).

  • Flow Cytometry - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)
    Flow Cytometry - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)

    Flow cytometric analysis of bEnd.3 (mouse brain endothelioma cell line) cells labeling LYVE1 with ab218535 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on total viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218535).

  • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)
    Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling LYVE1 with ab218535 at 1/5000 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining of the endothelium of lymph vessels in the submucosae on mouse stomach tissue section (PMID: 15705793).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218535).

  • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)
    Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling LYVE1 with ab218535 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining of the endothelium of sinusoid blood vessels on mouse liver tissue section (PMID: 11719431).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218535).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling LYVE1 with ab218535 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the lymphatic endothelium of mouse colon (PMID: 14722766). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218535).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)
    Anti-LYVE1 antibody [EPR21771] - BSA and Azide free (ab230377)

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