Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LIM Kinase 1 antibody [EPR912] - BSA and Azide free (ab239932)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR912] to LIM Kinase 1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-LIM Kinase 1 antibody [EPR912] - BSA and Azide free
    See all LIM Kinase 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR912] to LIM Kinase 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab239932 is the carrier-free version of ab108507 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239932 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LIM Kinase 1 antibody [EPR912] - BSA and Azide free (ab239932)
    Western blot - Anti-LIM Kinase 1 antibody [EPR912] - BSA and Azide free (ab239932)

    This data was developed using ab108507, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (40 µg)

    Lane 2: LIMK1 knockout HAP1 cell lysate (40 µg)

    Lane 3: PC12 cell lysate (20 µg)

    Lane 4: NIH3T3 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab108507 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab108507 was shown to specifically react with LIM Kinase 1 when LIM Kinase 1 knockout samples were used. Wild-type and LIM Kinase 1 knockout samples were subjected to SDS-PAGE. Ab108507 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-LIM Kinase 1 antibody [EPR912] - BSA and Azide free (ab239932)
    Western blot - Anti-LIM Kinase 1 antibody [EPR912] - BSA and Azide free (ab239932)
    All lanes : Anti-LIM Kinase 1 antibody [EPR912] (ab108507) at 1/1000 dilution

    Lane 1 : U-87 MG cell lysate
    Lane 2 : SH SY5Y cell lysate
    Lane 3 : Ramos cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 73 kDa



    This data was developed using ab108507, the same antibody clone in a different buffer formulation.

     

  • Anti-LIM Kinase 1 antibody [EPR912] - BSA and Azide free (ab239932)
    Anti-LIM Kinase 1 antibody [EPR912] - BSA and Azide free (ab239932)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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