Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)
![Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)](https://www.abcam.com/ps/products/119/ab119084/Images/LIM-Kinase-1-Primary-antibodies-ab119084-1.jpg "Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)")
Key features and details
- Mouse monoclonal [OTI3G3] to LIM Kinase 1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-LIM Kinase 1 antibody [OTI3G3]
See all LIM Kinase 1 primary antibodies -
Description
Mouse monoclonal [OTI3G3] to LIM Kinase 1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human LIM Kinase 1. Produced in HEK-293T cells. NP_002305.
Database link: P53667 -
Positive control
- WB: HEK-293T cells transfected with LIM Kinase 1. ICC/IF: COS-7 cells transiently transfected with LIM Kinase 1. IHC-P: Human endometrium adenocarcinoma and lymphoma tissue.
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General notes
The clone number has been updated from 3G3 to OTI3G3, both clone numbers name the same clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI3G3 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)All lanes : Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected the pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected the pCMV6-ENTRY LIM Kinase 1
Lysates/proteins at 5 µg per lane.
Predicted band size: 73 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)](https://www.abcam.com/ps/products/119/ab119084/Images/LIM-Kinase-1-Primary-antibodies-ab119084-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)Immunofluorescent staining of COS-7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1 using ab119084 at a dilution of 1/100.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)](https://www.abcam.com/ps/products/119/ab119084/Images/ab119084-314181-ab119084ihc2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human endometrium adenocarcinoma tissue using ab119084 at a 1/150 dilution. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)](https://www.abcam.com/ps/products/119/ab119084/Images/ab119084-314179-ab119084ihc1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human lymphoma tissue using ab119084 at a 1/150 dilution. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
