Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)
Key features and details
- Rabbit polyclonal to LIM Kinase 1 (phospho T508)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LIM Kinase 1 (phospho T508) antibody
See all LIM Kinase 1 primary antibodies -
Description
Rabbit polyclonal to LIM Kinase 1 (phospho T508) -
Host species
Rabbit -
Specificity
This antibody is specific for LIM Kinase only when phosphorylated at threonine 508. It recognizes both LIMK1 and LIMK2 from human, mouse, and rat. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide derived from Human LIM Kinase around the phosphorylation site of threonine 508.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)ab38508 at a 1:50 dilution staining LIM Kinase in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : untreated.
Right image : treated with phosphopeptide.
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Western blot - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)All lanes : Anti-LIM Kinase 1 (phospho T508) antibody (ab38508) at 1/500 dilution
Lane 1 : Extract of COLO cells + peptide.
Lane 2 : Extract of COLO cells.
Lane 3 : Extract of MCF7 cells.
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size: 73 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Lanes can be loaded with 5-30µg of total protein. The arrow next to the image points to LIM Kinase (phospho T508). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508) Image from Mokalled MH et al, Development. 2010 Jul;137(14):2365-74. Epub 2010 Jun 9, Fig 6. DOI: 10.1242/dev.047605.ab38508 staining LIM Kinase 1 in mouse brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
bdKO mice were anesthetized and transcardially perfused with PBS, followed by 4% paraformaldehyde (PFA) prior to brain dissection. Brains were then postfixed in 4% PFA for 2 days, embedded in paraffin and sectioned.
