Anti-LIM Kinase 1 antibody [EPR912] (ab108507)
![Anti-LIM Kinase 1 antibody [EPR912] (ab108507)](https://www.abcam.com/ps/products/108/ab108507/Images/ab108507-268427-anti-lim-kinase-1-antibody-epr912-western-blot.jpg "Anti-LIM Kinase 1 antibody [EPR912] (ab108507)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR912] to LIM Kinase 1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LIM Kinase 1 antibody [EPR912]
See all LIM Kinase 1 primary antibodies -
Description
Rabbit monoclonal [EPR912] to LIM Kinase 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- U-87 MG, SH SY5Y and Ramos cell lysates.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR912 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LIM Kinase 1 antibody [EPR912] (ab108507)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: LIMK1 knockout HAP1 cell lysate (40 µg)
Lane 3: PC12 cell lysate (20 µg)
Lane 4: NIH3T3 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108507 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.ab108507 was shown to specifically react with LIM Kinase 1 when LIM Kinase 1 knockout samples were used. Wild-type and LIM Kinase 1 knockout samples were subjected to SDS-PAGE. Ab108507 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-LIM Kinase 1 antibody [EPR912] (ab108507)](https://www.abcam.com/ps/products/108/ab108507/Images/ab108507-121205-anti-lim-kinase-1-antibody-epr912-western-blot.jpg)
Western blot - Anti-LIM Kinase 1 antibody [EPR912] (ab108507)All lanes : Anti-LIM Kinase 1 antibody [EPR912] (ab108507) at 1/1000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa
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![Anti-LIM Kinase 1 antibody [EPR912] (ab108507)](https://www.abcam.com/ps/products/108/ab108507/Images/ab108507-3-benefits-of-recombinant-antibodies.png)
Anti-LIM Kinase 1 antibody [EPR912] (ab108507)
