Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18791] to IGF2BP1/IMP1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-IGF2BP1/IMP1 antibody [EPR18791]
See all IGF2BP1/IMP1 primary antibodies -
Description
Rabbit monoclonal [EPR18791] to IGF2BP1/IMP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293, HeLa and K562 whole cell lysates; Human fetal liver and fetal kidney tissue lysates. IP: K562 whole cell lysate. IHC-P: Human testis, tonsil, Hodgkin's lymphoma and lung cancer tissues.
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General notes
This product was previously labelled as IGF2BP1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18791 -
Isotype
IgG -
Research areas
Images
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Lane 1 : Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305) at 1/5000 dilution
Lanes 2-4 : Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305) at 1/1000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 63 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?Exposure times: Lane 1: 8 seconds; Lanes 2,3: 15 seconds; Lane 4: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
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IGF2BP1/IMP1 was immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab184305 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184305 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: K562 whole cell lysate 10 μg (Input).
Lane 2: ab184305 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184305 in K562 whole cell lysate.Exposure time: 1 second.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Faint, lower molecular mass bands are observed in some human cell lines including K562. These could be an isoform (Isoform 2 predicted to be 48.5kDa) and degradation products, as described in the literature (PMID: 11641779).
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human lung cancer (PMID: 17255263) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining in human Hodgkin’s lymphoma (PMID: 17296566) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in germinal center of human tonsil (PMID: 17296566) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human testis tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human testis (PMID: 16049158) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Lane 1 : Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305) at 1/10000 dilution
Lanes 2-3 : Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 63 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?Exposure times: Lane 1: 102 seconds; Lanes 2,3: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
Faint, lower molecular mass bands are observed in some human cell lines including K562. These could be an isoform (Isoform 2 predicted to be 48.5kDa) and degradation products, as described in the literature (PMID: 11641779). The BxPC-3 and MCF7 cell lines are negative controls (PMID 26917013).
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