Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18791] to IGF2BP1/IMP1 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free
    See all IGF2BP1/IMP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18791] to IGF2BP1/IMP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lung cancer tissue.

  • General notes

    ab229700 is the carrier-free version of ab184305.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)
    Immunoprecipitation - Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)

    IGF2BP1/IMP1 was immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab184305 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184305 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: K562 whole cell lysate 10 μg (Input).
    Lane 2: ab184305 IP in K562 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184305 in K562 whole cell lysate.

    Exposure time: 1 second.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Faint, lower molecular mass bands are observed in some human cell lines including K562. These could be an isoform (Isoform 2 predicted to be 48.5kDa) and  degradation products, as described in the literature (PMID: 11641779).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184305).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)

    Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining in human Hodgkin’s lymphoma (PMID: 17296566) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184305).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in germinal center of human tonsil (PMID: 17296566) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184305).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human testis (PMID: 16049158) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184305).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human lung cancer (PMID: 17255263) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184305).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)
    Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)

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