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Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free (ab264063)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [SMab-2] to IDH2 (mutated R172S) - BSA and Azide free
  • Suitable for: WB, ICC/IF, ELISA
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free
    See all IDH2 primary antibodies

  • Description

    Mouse monoclonal [SMab-2] to IDH2 (mutated R172S) - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, ELISAmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein. ICC/IF: SW1353 cells.

  • General notes

    ab264063 is the carrier-free version of ab264056. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab264063 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free (ab264063)
    Western blot - Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free (ab264063)

    Western blot - Anti-IDH2 (mutated R172 S) antibody [SMab-2] (ab264056).

    The loading samples are E.coil extracts containing recombinant protein respectively.

    ab264056 used at a 1/1000 dilution (1.329µg/ml), followed by Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) secondary antibody at a 1/10,000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein 10ng
    Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
    Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
    Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
    Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
    Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
    Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
    Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein 10ng
    Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
    Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
    Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
    Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
    Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
    Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264056).

  • Immunocytochemistry/ Immunofluorescence - Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free (ab264063)
    Immunocytochemistry/ Immunofluorescence - Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free (ab264063)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW1353 (human chondrosarcoma cell line) cells labelling IDH2 (mutated R172 S) with ab264056 at 1/50 dilution (26.58µg/ml), followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SW1353 cells.

    Negative control: HeLa (PMID: 25753205).

    ab179504 (YCA-R16775) Anti-beta IV Tubulin antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab264056 at a 1/50 dilution (26.58µg/ml) followed by ab150080 at a 1/1000 dilution.

    Negative control 2: ab179504 at 1/200 dilution followed by ab150113 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264056).

  • ELISA - Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free (ab264063)
    ELISA - Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free (ab264063)

    ELISA - Anti-IDH2 (mutated R172 S) antibody [SMab-2] (ab264056).

    ab264056 used at 0-5000 ng/ml, followed by an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) secondary used at 1/1000 dilution.

    Antigen concentration, 100 ng/ml.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264056).

  • Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free (ab264063)
    Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free (ab264063)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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