Ionomycin (free acid), Ca2+ ionophore (ab120370)
Key features and details
- Ca2+ ionophore
- CAS Number: 56092-81-0
- Purity: > 97%
- Soluble in ethanol to 10 mM and in DMSO to 10 mM
- Form / State: Liquid
- Source: Streptomyces conglobatus
Overview
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Product name
Ionomycin (free acid), Ca2+ ionophore -
Description
Ca2+ ionophore -
Biological description
Potent, highly selective Ca2+ ionophore. Effective mobile ion carrier for Ca2+, highly specific for divalent cations (Ca > Mg >> Sr = Ba). Useful for studies of Ca2+ transport across biological membranes and increasing intracellular Ca2+ concentrations. Complexes with Ca2+ between pH 7 and 9.5. Absorption max. (280 nm, MeOH): 18600 M-1cm-1.
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Purity
> 97% -
CAS Number
56092-81-0 -
Chemical structure
Images
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Functional Studies - Ionomycin (free acid), Ca2+ ionophore (ab120370)ab58668 staining ATF3 in A549 cells treated with ionomycin (free acid) (ab120370), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin (free acid), as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120370 (Ionomycin (free acid)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.