Anti-MOCS2 antibody (ab230229)
Key features and details
- Rabbit polyclonal to MOCS2
- Suitable for: IP, IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MOCS2 antibody
See all MOCS2 primary antibodies -
Description
Rabbit polyclonal to MOCS2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human MOCS2 aa 1 to the C-terminus.
Database link: O96007 -
Positive control
- WB: A549, HepG2, Jurkat and HeLa whole cell lysates. IHC-P: Human kidney and adrenal gland tissues. IP: Jurkat whole cell lysate. ICC/IF: HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-MOCS2 antibody (ab230229)All lanes : Anti-MOCS2 antibody (ab230229) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21,49 kDa why is the actual band size different from the predicted?
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCS2 antibody (ab230229)Paraffin-embedded human kidney tissue stained for MOCS2 using ab230229 at 1/100 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCS2 antibody (ab230229)Paraffin-embedded human adrenal gland tissue stained for MOCS2 using ab230229 at 1/100 dilution in immunohistochemical analysis.
-
Immunoprecipitation - Anti-MOCS2 antibody (ab230229)MOCS2 was immunoprecipitated from 500 µg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with 4 µg ab230229.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in Jurkat whole cell lysate.
Lane 2: ab230229 IP in Jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-MOCS2 antibody (ab230229)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MOCS2 (green) using ab230229 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
