Anti-MOCS2 antibody (ab230013)
Key features and details
- Rabbit polyclonal to MOCS2
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MOCS2 antibody
See all MOCS2 primary antibodies -
Description
Rabbit polyclonal to MOCS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Recombinant full length protein corresponding to Human MOCS2 aa 1 to the C-terminus.
Database link: O96007 -
Positive control
- WB: A549, HepG2 and Jurkat whole cell lysatses. IHC-P: Human kidney and adrenal gland tissues. IP: Jurkat whole cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MOCS2 antibody (ab230013)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MOCS2 (green) using ab230013 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
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Immunoprecipitation - Anti-MOCS2 antibody (ab230013)MOCS2 was immunoprecipitated from 500 µg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with 4 µg of ab230013.
Lane 1: 1 µg of rabbit monoclonal IgG instead of ab230013 in Jurkat whole cell lysate.
Lane 2: ab230013 IP in jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate 20 µg (input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCS2 antibody (ab230013)Paraffin-embedded human adrenal gland tissue stained for MOCS2 using ab230013 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MOCS2 antibody (ab230013)Paraffin-embedded human kidney tissue stained for ab230013 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-MOCS2 antibody (ab230013)All lanes : Anti-MOCS2 antibody (ab230013) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
