Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GAA antibody [EPR4716(2)] (ab137068)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4716(2)] to GAA
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GAA antibody [EPR4716(2)]
    See all GAA primary antibodies

  • Description

    Rabbit monoclonal [EPR4716(2)] to GAA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GAA aa 150-250. The exact sequence is proprietary.
    Database link: P10253

  • Positive control

    • WB: HEK-293T whole cell lysate. Human fetal liver, placenta, liver, brain, and thymus tissue lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GAA antibody [EPR4716(2)] (ab137068)
    Western blot - Anti-GAA antibody [EPR4716(2)] (ab137068)
    All lanes : Anti-GAA antibody [EPR4716(2)] (ab137068) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : Human fetal liver tissue lysate
    Lane 3 : Human placenta tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 105 kDa
    Observed band size: 110,75,95 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-GAA antibody [EPR4716(2)] (ab137068)
    Western blot - Anti-GAA antibody [EPR4716(2)] (ab137068)
    Anti-GAA antibody [EPR4716(2)] (ab137068) at 1/1000 dilution + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 105 kDa


    Exposure time: 20 seconds
  • Western blot - Anti-GAA antibody [EPR4716(2)] (ab137068)
    Western blot - Anti-GAA antibody [EPR4716(2)] (ab137068)
    All lanes : Anti-GAA antibody [EPR4716(2)] (ab137068) at 1/1000 dilution

    Lane 1 : Human liver lysates
    Lane 2 : Human brain lysates
    Lane 3 : Human thymus lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 105 kDa
    Observed band size: 110,75,95 kDa why is the actual band size different from the predicted?



    Exposure time:

    Lane 1: 10 seconds
    Lane 2: and 3: 30 seconds

  • Anti-GAA antibody [EPR4716(2)] (ab137068)
    Anti-GAA antibody [EPR4716(2)] (ab137068)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-GAA antibody [EPR4716(2)] (ab137068)

© 2021 Astana Biomed Group LLP. All rights reserved.