Anti-GAA antibody [EPR4716(2)] (ab137068)
![Anti-GAA antibody [EPR4716(2)] (ab137068)](https://www.abcam.com/ps/products/137/ab137068/Images/ab137068-151599-anti-gaa-antibody-epr47162-western-blot.jpg "Anti-GAA antibody [EPR4716(2)] (ab137068)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4716(2)] to GAA
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-GAA antibody [EPR4716(2)]
See all GAA primary antibodies -
Description
Rabbit monoclonal [EPR4716(2)] to GAA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human GAA aa 150-250. The exact sequence is proprietary.
Database link: P10253 -
Positive control
- WB: HEK-293T whole cell lysate. Human fetal liver, placenta, liver, brain, and thymus tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4716(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GAA antibody [EPR4716(2)] (ab137068)All lanes : Anti-GAA antibody [EPR4716(2)] (ab137068) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Human placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 105 kDa
Observed band size: 110,75,95 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-GAA antibody [EPR4716(2)] (ab137068)](https://www.abcam.com/ps/products/137/ab137068/Images/ab137068-356624-anti-gaa-antibody-epr4716-2-westernblot-human-hek293.jpg)
Western blot - Anti-GAA antibody [EPR4716(2)] (ab137068)Anti-GAA antibody [EPR4716(2)] (ab137068) at 1/1000 dilution + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 105 kDa
Exposure time: 20 seconds
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![Western blot - Anti-GAA antibody [EPR4716(2)] (ab137068)](https://www.abcam.com/ps/products/137/ab137068/Images/ab137068-356623-anti-gaa-antibody-epr4716-2-westernblot-human-liver-brain-tyomus.jpg)
Western blot - Anti-GAA antibody [EPR4716(2)] (ab137068)All lanes : Anti-GAA antibody [EPR4716(2)] (ab137068) at 1/1000 dilution
Lane 1 : Human liver lysates
Lane 2 : Human brain lysates
Lane 3 : Human thymus lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 105 kDa
Observed band size: 110,75,95 kDa why is the actual band size different from the predicted?Exposure time:
Lane 1: 10 seconds
Lane 2: and 3: 30 seconds -
![Anti-GAA antibody [EPR4716(2)] (ab137068)](https://www.abcam.com/ps/products/137/ab137068/Images/ab137068-4-benefits-of-recombinant-antibodies.png)
Anti-GAA antibody [EPR4716(2)] (ab137068)
