Anti-HNF6 antibody [EPR14976] (ab186743)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14976] to HNF6
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-HNF6 antibody [EPR14976]
See all HNF6 primary antibodies -
Description
Rabbit monoclonal [EPR14976] to HNF6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver, human and rat liver tissue lysates; HepG2 and Hepa1-6 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14976 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-HNF6 antibody [EPR14976] (ab186743) at 1/200 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2 : Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysates at 15 µg
Lane 3 : Rat liver tissue lysates at 15 µg
Lane 4 : Human liver tissue lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 100 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
This antibody shows low affinity on liver samples which should be positive according to PMID 27445394.
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