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Signal Transduction Metabolism Energy Metabolism

Anti-HNF6 antibody (ab69310)

Price and availability

244 579 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-HNF6 antibody (ab69310)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to HNF6
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HNF6 antibody
    See all HNF6 primary antibodies
  • Description

    Rabbit polyclonal to HNF6
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human HNF6 aa 350-450 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90165)

  • Positive control

    • Purchase matching WB positive control:Recombinant Human HNF6 protein
    • This antibody gave a positive signal in human liver tissue lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Liver development
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Pancreas development
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab69310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Cow
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • Function

    Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
  • Tissue specificity

    Highly expressed in liver; lower expression in testis and skin.
  • Sequence similarities

    Belongs to the CUT homeobox family.
    Contains 1 CUT DNA-binding domain.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9UBC0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3175 Human
    • Entrez Gene: 15379 Mouse
    • Entrez Gene: 25231 Rat
    • Omim: 604164 Human
    • SwissProt: Q9UBC0 Human
    • SwissProt: O08755 Mouse
    • SwissProt: P70512 Rat
    • Unigene: 658573 Human
    • Unigene: 303355 Mouse
    • Unigene: 48812 Rat
    see all
  • Alternative names

    • hepatocyte nuclear factor 6 alpha antibody
    • Hepatocyte nuclear factor 6 antibody
    • HNF 6 antibody
    • HNF-6 antibody
    • HNF6_HUMAN antibody
    • HNF6A antibody
    • One cut domain family member 1 antibody
    • One cut homeobox 1 antibody
    • Onecut1 antibody
    see all

Images

  • Western blot - Anti-HNF6 antibody (ab69310)
    Western blot - Anti-HNF6 antibody (ab69310)
    Anti-HNF6 antibody (ab69310) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa
    Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab69310? Please let us know so that we can cite the reference in this datasheet.

    ab69310 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-HNF6 antibody (ab69310)
      Western blot - Anti-HNF6 antibody (ab69310)
      Anti-HNF6 antibody (ab69310) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 51 kDa
      Observed band size: 51 kDa
      Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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