Anti-HNF6 antibody (ab69310)
Key features and details
- Rabbit polyclonal to HNF6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-HNF6 antibody
See all HNF6 primary antibodies -
Description
Rabbit polyclonal to HNF6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide corresponding to Human HNF6 aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90165) -
Positive control
- Purchase matching WB positive control:Recombinant Human HNF6 protein
- This antibody gave a positive signal in human liver tissue lysate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentrationConcentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).Target
-
Function
Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. -
Tissue specificity
Highly expressed in liver; lower expression in testis and skin. -
Sequence similarities
Belongs to the CUT homeobox family.
Contains 1 CUT DNA-binding domain.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 3175 Human
- Entrez Gene: 15379 Mouse
- Entrez Gene: 25231 Rat
- Omim: 604164 Human
- SwissProt: Q9UBC0 Human
- SwissProt: O08755 Mouse
- SwissProt: P70512 Rat
- Unigene: 658573 Human
see all -
Alternative names
- hepatocyte nuclear factor 6 alpha antibody
- Hepatocyte nuclear factor 6 antibody
- HNF 6 antibody
see all
Images
-
Anti-HNF6 antibody (ab69310) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Datasheets and documents
References (0)
ab69310 has not yet been referenced specifically in any publications.
Images
-
Anti-HNF6 antibody (ab69310) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute