Anti-TLR3 antibody (ab62567)
Key features and details
- Rabbit polyclonal to TLR3
- Suitable for: ICC, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TLR3 antibody
See all TLR3 primary antibodies -
Description
Rabbit polyclonal to TLR3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human TLR3 aa 40-90 (N terminal). The exact sequence is proprietary. 15 aa long.
Database link: O15455 -
Positive control
- ICC/IF: K562 cells. WB: EL4 and K562 cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-TLR3 antibody (ab62567) at 0.5 µg/ml + EL4 (mouse lymphoma cell line) cell lysate
Predicted band size: 104 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
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Lane 1 : Anti-TLR3 antibody (ab62567) at 0.5 µg/ml
Lane 2 : Anti-TLR3 antibody (ab62567) at 1 µg/ml
Lane 3 : Anti-TLR3 antibody (ab62567) at 2 µg/ml
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lanes 2-3 : K562 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 104 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
There are multiple additional bands. We are unsure as to the identity of these extra bands. -
Immunofluorescence of TLR3 in 0.1% Triton permeabilized K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells using ab62567 at 2 µg/ml.
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ab62567, at 10µg/ml, staining TLR3 in K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells by immunocytochemistry. DAB detection. Counterstained with hematoxylin.