Anti-TLR3 antibody (ab137722)
Key features and details
- Rabbit polyclonal to TLR3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TLR3 antibody
See all TLR3 primary antibodies -
Description
Rabbit polyclonal to TLR3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cat -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 464-647 of Human TLR3.
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Positive control
- WB: K562 and THP-1 whole cell lysates; IHC-P: Human TOV-112D xenograft, mouse liver, rat pancreas tissue; IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft labelling TLR3 with ab137722 at 1/100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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All lanes : Anti-TLR3 antibody (ab137722) at 1/5000 dilution
Lane 1 : K562 whole cell lysate
Lane 2 : THP-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 104 kDa
5% SDS PAGE -
Immunofluorescenct analysis of methanol-fixed HeLa cells labelling TLR3 with ab137722 at 1/200 dilution. Lower image is co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling TLR3 at cytoplasm with ab137722 at 1/1000 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labelling TLR3 at cytoplasm with ab137722 at 1/1000 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.