Anti-TLR3 antibody (ab62566)
Key features and details
- Rabbit polyclonal to TLR3
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TLR3 antibody
See all TLR3 primary antibodies -
Description
Rabbit polyclonal to TLR3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC MouseHumanIHC-P MouseHumanWB MouseHuman
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Immunogen
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Positive control
- WB: Daudi cell lysate. IHC-P: Human and mouse spleen. ICC: EL4 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody (ab62566)
Paraffin-embedded mouse spleen tissue stained for TLR3 using ab62566 at 2 µg/ml in immunohistochemical analysis. DAB detection. Counterstained with hematoxylin.
Antigen Retrieval (Microwave Method).
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Western blot - Anti-TLR3 antibody (ab62566)Western blot analysis of TLR3 expression in mouse spleen tissue lysate with ab62566 at (A) 0.5 and (B) 1µg/ml. -
Western blot - Anti-TLR3 antibody (ab62566)Lane 1 : Anti-TLR3 antibody (ab62566) at 1 µg/ml
Lane 2 : Anti-TLR3 antibody (ab62566) at 2 µg/ml
All lanes : Daudi (Human Burkitt's lymphoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 104 kDa
Observed band size: 104 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody (ab62566)Paraffin-embedded human spleen tissue stained for TLR3 using ab62566 at 5 µg/ml in immunohistochemical analysis. DAB detection. Counterstained with hematoxylin.
Antigen Retrieval (Microwave Method).
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Immunocytochemistry - Anti-TLR3 antibody (ab62566)Immunofluorescence of TLR3 in 0.1% Triton X-100 permeabilized EL4 (mouse lymphoblast cell line) cells using ab62566 at 10 µg/ml.
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Immunocytochemistry - Anti-TLR3 antibody (ab62566)ab62566, at 1µg/ml, staining TLR3 in 0.1% Triton X-100 permeabilized EL4 (mouse lymphoblast cell line) cells by immunocytochemistry. DAB detection. Counterstained with hematoxylin.
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Immunocytochemistry - Anti-TLR3 antibody (ab62566)Immunofluorescence analysis of mouse spleen tissue labeling TLR3 using ab62566 at 20 µg/ml (green).
Antigen Retrieval (Microwave Method).
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Immunocytochemistry - Anti-TLR3 antibody (ab62566)Immunofluorescence analysis of human spleen tissue labeling TLR3 using ab62566 at 20 µg/ml (green).
Antigen Retrieval (Microwave Method).
