Anti-CTHRC1 antibody (ab85739)
Key features and details
- Rabbit polyclonal to CTHRC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CTHRC1 antibody
See all CTHRC1 primary antibodies -
Description
Rabbit polyclonal to CTHRC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human CTHRC1 aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab101727) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Images
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Western blot - Anti-CTHRC1 antibody (ab85739)All lanes : Anti-CTHRC1 antibody (ab85739) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
We predict that the 20 kDa band represents the cleaved form of CTHRC1. Abcam welcomes customer feedback. -
Immunocytochemistry/ Immunofluorescence - Anti-CTHRC1 antibody (ab85739)ICC/IF image of ab85739 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85739, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-CTHRC1 antibody (ab85739)All lanes : Anti-CTHRC1 antibody (ab85739) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human CTHRC1 peptide (ab101727) at 1 µg/ml
Lane 5 : Human heart tissue lysate - total protein (ab29431) with Human CTHRC1 peptide (ab101727) at 1 µg/ml
Lane 6 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989) with Human CTHRC1 peptide (ab101727) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody (ab85739)IHC image of CTHRC1 staining in human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85739, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
