Anti-CTHRC1 antibody (ab264410)
Key features and details
- Rabbit polyclonal to CTHRC1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CTHRC1 antibody
See all CTHRC1 primary antibodies -
Description
Rabbit polyclonal to CTHRC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human CTHRC1 aa 25-75. The exact sequence is proprietary.
Database link: Q96CG8 -
Positive control
- WB: UACC-62 whole cell lysate. IP: UACC-62 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CTHRC1 antibody (ab264410)All lanes : Anti-CTHRC1 antibody (ab264410) at 1/1000 dilution
Lane 1 : UACC-62 whole cell lysate
Lane 2 : MALME-3M whole cell lysate
Lane 3 : SR whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-CTHRC1 antibody (ab264410)CTHRC1 was immunoprecipitated from UACC-62 whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264410 at 100 µl per reaction. Western blot was performed from the immunoprecipitate using an alternative anti-CTHRC1 antibody
Lane 1: ab264410 IP in UACC-62 whole cell lysate.
Lane 2: Control IgG IP in UACC-62 whole cell lysate.Detection: Chemiluminescence with an exposure time of 1 second.
Lysates prepared using NETN lysis buffer.
