Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Adenosine A1 Receptor antibody (ab82477)

Key features and details

  • Rabbit polyclonal to Adenosine A1 Receptor
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Overview

  • Product name

    Anti-Adenosine A1 Receptor antibody
    See all Adenosine A1 Receptor primary antibodies

  • Description

    Rabbit polyclonal to Adenosine A1 Receptor

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to Rat Adenosine A1 Receptor aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab91076)

  • Positive control

    • This antibody gave a positive signal in Rat Spinal Ganglion Neuronal cell lysate and Mouse EL4 Lymphoma whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • Western blot - Anti-Adenosine A1 Receptor antibody (ab82477)
    Western blot - Anti-Adenosine A1 Receptor antibody (ab82477)
    All lanes : Anti-Adenosine A1 Receptor antibody (ab82477) at 1 µg/ml

    Lane 1 : Rat Spinal Ganglion Neuronal cell lysate
    Lane 2 : EL4 whole cell lysate (ab7183)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa
    Additional bands at: 118 kDa, 18 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • Immunocytochemistry/ Immunofluorescence - Anti-Adenosine A1 Receptor antibody (ab82477)
    Immunocytochemistry/ Immunofluorescence - Anti-Adenosine A1 Receptor antibody (ab82477)
    ICC/IF image of ab82477 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82477, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (ab82477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (ab82477)
    IHC image of ab82477 staining in Rat Adult Brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82477, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References (14)

Publishing research using ab82477? Please let us know so that we can cite the reference in this datasheet.

ab82477 has been referenced in 14 publications.

  • Han YY  et al. Cordycepin improves behavioral-LTP and dendritic structure in hippocampal CA1 area of rats. J Neurochem 151:79-90 (2019). PubMed: 31314908
  • Tian D  et al. The effect of A1 adenosine receptor in diabetic megalin loss with caspase-1/IL18 signaling. Diabetes Metab Syndr Obes 12:1583-1596 (2019). PubMed: 31695457
  • Liu Z  et al. Amyloid ß and tau are involved in sleep disorder in Alzheimer's disease by orexin A and adenosine A(1) receptor. Int J Mol Med 43:435-442 (2019). PubMed: 30365112
  • Huang W  et al. An adenosine A1R-A2aR imbalance regulates low glucose/hypoxia-induced microglial activation, thereby contributing to oligodendrocyte damage through NF-?B and CREB phosphorylation. Int J Mol Med 41:3559-3569 (2018). PubMed: 29512780
  • Navarro G  et al. Cross-communication between Gi and Gs in a G-protein-coupled receptor heterotetramer guided by a receptor C-terminal domain. BMC Biol 16:24 (2018). PubMed: 29486745
View all Publications for this product

Images

  • Western blot - Anti-Adenosine A1 Receptor antibody (ab82477)
    Western blot - Anti-Adenosine A1 Receptor antibody (ab82477)
    All lanes : Anti-Adenosine A1 Receptor antibody (ab82477) at 1 µg/ml

    Lane 1 : Rat Spinal Ganglion Neuronal cell lysate
    Lane 2 : EL4 whole cell lysate (ab7183)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa
    Additional bands at: 118 kDa, 18 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • Immunocytochemistry/ Immunofluorescence - Anti-Adenosine A1 Receptor antibody (ab82477)
    Immunocytochemistry/ Immunofluorescence - Anti-Adenosine A1 Receptor antibody (ab82477)
    ICC/IF image of ab82477 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82477, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (ab82477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (ab82477)
    IHC image of ab82477 staining in Rat Adult Brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82477, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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