Recombinant human IL2 Receptor beta/p75 protein (Active) (ab174003)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Biological Activity, ELISA, Functional Studies
Preparation and Storage
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Images
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Biological activity - Recombinant human IL2 Receptor beta/p75 protein (ab174003)
ab174003 inhibits the IL-15-dependent proliferation of Mo7e cells. The EC50 for this effect is 0.38-0.45 µg/mL.
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Functional Studies - Recombinant human IL2 Receptor beta/p75 protein (ab174003)Loaded ab174003 on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 0.46 μM as determined in BLI assay (ForteBio Octet Red96e).
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Functional Studies - Recombinant human IL2 Receptor beta/p75 protein (ab174003)ab174003 captured on CM5 chip via anti-His antibody, can bind Human IL-15, Tag Free with an affinity constant of 21.7 nM as determined in a SPR assay (Biacore T200).
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Functional Studies - Recombinant human IL2 Receptor beta/p75 protein (ab174003)ab174003 captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 0.525 µM as determined in a SPR assay (Biacore T200).
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SDS-PAGE - Recombinant human IL2 Receptor beta/p75 protein (ab174003)SDS-PAGE of reduced ab174003 stained overnight with Coomassie Blue. The protein migrates as 35-45 kDa due to glycosylation.
