Anti-Bcl-2 (phospho S87) antibody (ab73985)
Key features and details
- Rabbit polyclonal to Bcl-2 (phospho S87)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bcl-2 (phospho S87) antibody
See all Bcl-2 primary antibodies -
Description
Rabbit polyclonal to Bcl-2 (phospho S87) -
Host species
Rabbit -
Specificity
ab73985 detects endogenous levels of Bcl-2 only when phosphorylated at serine 87 (Human: Ser87; Mouse: Ser84; Rat: Ser84). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Bcl-2.
Database link: P10415
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab73985 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bcl-2 (phospho S87) antibody (ab73985)All lanes : Anti-Bcl-2 (phospho S87) antibody (ab73985) at 1/500 dilution
Lane 1 : Extracts from HeLa cells, treated
with nocodazole (1ug/ml, 18hours)
Lane 2 : Extracts from HeLa cells, treated
with nocodazole (1ug/ml, 18hours) with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 26 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 (phospho S87) antibody (ab73985)Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using ab73985 at 1/50 dilution in the presence (right panel) or absence (left panel) of immunising phosphopeptide.
