Anti-6X His tag® antibody (ab9136)
Key features and details
- Goat polyclonal to 6X His tag®
- Suitable for: ELISA, ICC, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
Goat polyclonal to 6X His tag® -
Host species
Goat -
Specificity
This antibody was verified by ELISA against peptide conjugated to BSA(HHHHHH/BSA).
ab9136 does not react to all 6X His tag® fused to amino terminus proteins.
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Tested applications
Suitable for: ELISA, ICC, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-6X His tag® antibody (ab9136)All lanes : Anti-6X His tag® antibody (ab9136) at 1/5000 dilution
Lane 1 : E.coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E.coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E.coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Developed using the ECL technique.
Exposure time: 3 minutes
