Anti-6X His tag® antibody (ab14923)
Key features and details
- Rabbit polyclonal to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
Rabbit polyclonal to 6X His tag® -
Host species
Rabbit -
Specificity
ab14923 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus in transfected or transformed cells.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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Positive control
- E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein, ab2431.
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB (1) Use a concentration of 0.5 µg/ml.Notes WB
Use a concentration of 0.5 µg/ml.Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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Western blot - Anti-6X His tag® antibody (ab14923)Anti-6X His tag® antibody (ab14923) at 0.6 µg/ml +
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 17 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (8)
ab14923 has been referenced in 8 publications.
- Sun J et al. Histone demethylase LSD1 regulates bone mass by controlling WNT7B and BMP2 signaling in osteoblasts. Bone Res 6:14 (2018). PubMed: 29707403
- Shao Q et al. Uncoupling of UNC5C with Polymerized TUBB3 in Microtubules Mediates Netrin-1 Repulsion. J Neurosci 37:5620-5633 (2017). PubMed: 28483977
- Pokidysheva E et al. Biological role of prolyl 3-hydroxylation in type IV collagen. Proc Natl Acad Sci U S A 111:161-6 (2014). PubMed: 24368846
- Knapp RT et al. Hsp70 cochaperones HspBP1 and BAG-1M differentially regulate steroid hormone receptor function. PLoS One 9:e85415 (2014). WB . PubMed: 24454860
- Giegerich AK et al. Autophagy-dependent PELI3 degradation inhibits proinflammatory IL1B expression. Autophagy 10:1937-52 (2014). PubMed: 25483963
- Loza-Correa M et al. The Legionella pneumophila kai operon is implicated in stress response and confers fitness in competitive environments. Environ Microbiol 16:359-81 (2014). PubMed: 23957615
- Zhang D et al. The Chromatin-Remodeling Factor PICKLE Integrates Brassinosteroid and Gibberellin Signaling during Skotomorphogenic Growth in Arabidopsis. Plant Cell 26:2472-2485 (2014). PubMed: 24920333
- Abdelal AT et al. Ornithine transcarbamylase from Salmonella typhimurium: purification, subunit composition, kinetic analysis, and immunological cross-reactivity. J Bacteriol 129:1387-96 (1977). PubMed: 14923
Images
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Western blot - Anti-6X His tag® antibody (ab14923)Anti-6X His tag® antibody (ab14923) at 0.6 µg/ml +
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 17 kDa why is the actual band size different from the predicted?
