Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µg 274 732 ₸

Order now and get it on Thursday February 25, 2021

Key features and details

Product name

Anti-6X His tag® antibody
See all 6X His tag® primary antibodies
Description

Rabbit polyclonal to 6X His tag®
Host species

Rabbit
Specificity

ab14923 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus in transfected or transformed cells.
Tested applications

Suitable for: WBmore details
Species reactivity

Reacts with: Species independent
Immunogen

Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.
(Peptide available as ab14941, ab14943)
Positive control
- E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein, ab2431.
General notes

HIS-TAG^® is a trademark of EMD Biosciences, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

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Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab14923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	Use a concentration of 0.5 µg/ml.

Notes
WB Use a concentration of 0.5 µg/ml.

Relevance

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localization

Depends upon the localization of the parent protein tagged with hexahistidine.
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
- HHHHHH tag antibody
- His tag antibody
- Polyhistidine Tag antibody
see all

Publishing research using ab14923? Please let us know so that we can cite the reference in this datasheet.

ab14923 has been referenced in 8 publications.

Sun J et al. Histone demethylase LSD1 regulates bone mass by controlling WNT7B and BMP2 signaling in osteoblasts. Bone Res 6:14 (2018). PubMed: 29707403
Shao Q et al. Uncoupling of UNC5C with Polymerized TUBB3 in Microtubules Mediates Netrin-1 Repulsion. J Neurosci 37:5620-5633 (2017). PubMed: 28483977
Pokidysheva E et al. Biological role of prolyl 3-hydroxylation in type IV collagen. Proc Natl Acad Sci U S A 111:161-6 (2014). PubMed: 24368846
Knapp RT et al. Hsp70 cochaperones HspBP1 and BAG-1M differentially regulate steroid hormone receptor function. PLoS One 9:e85415 (2014). WB . PubMed: 24454860
Giegerich AK et al. Autophagy-dependent PELI3 degradation inhibits proinflammatory IL1B expression. Autophagy 10:1937-52 (2014). PubMed: 25483963
Loza-Correa M et al. The Legionella pneumophila kai operon is implicated in stress response and confers fitness in competitive environments. Environ Microbiol 16:359-81 (2014). PubMed: 23957615
Zhang D et al. The Chromatin-Remodeling Factor PICKLE Integrates Brassinosteroid and Gibberellin Signaling during Skotomorphogenic Growth in Arabidopsis. Plant Cell 26:2472-2485 (2014). PubMed: 24920333
Abdelal AT et al. Ornithine transcarbamylase from Salmonella typhimurium: purification, subunit composition, kinetic analysis, and immunological cross-reactivity. J Bacteriol 129:1387-96 (1977). PubMed: 14923