Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate, Urine
Overview
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Product name
Catalase Activity Assay Kit (Colorimetric/Fluorometric)
See all Catalase kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Urine, Serum, Plasma, Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Assay time
0h 40m -
Species reactivity
Reacts with: Mammals -
Product overview
Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.
In the catalase activity assay protocol, the catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 535/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 µU of catalase activity.
Catalase activity assay protocol summary:
- add samples and standards to wells
- add stop solution into sample control wells and incubate at 25ºC for 5 min
- add H2O2 solution into wells and incubate for 30 min at 25ºC
- add stop solution
- add developer mix and incubate for 10 min at 25ºC
- analyze with microplate reader -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 100 tests Catalase Assay Buffer NM 1 x 25ml Catalase Positive Control Blue 1 x 2µl HRP (Lyophilised) Green 1 vial Hydrogen peroxide Yellow 1 x 25µl OxiRed Probe Red 1 x 200µl Stop Solution White 1 x 1ml -
Research areas
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Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
Sequence similarities
Belongs to the catalase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Peroxisome. - Information by UniProt
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Alternative names
- Cas1
- CAT
- CATA_HUMAN
see all
Images
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Functional Studies - Catalase Assay Kit (ab83464)Sample tests using ab83464.
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Functional Studies - Catalase Assay Kit (ab83464)H2O2 standard curve using ab83464.
