Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free
See all Bcl G/BCL2L14 primary antibodies
Description

Rabbit monoclonal [EPR17666] to Bcl G/BCL2L14 - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
ab250782 is the carrier-free version of ab184925 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250782 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as Bcl G
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17666
Isotype

IgG
Research areas

Immunocytochemistry - Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free (ab250782)

This data was developed using ab184925, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Bcl G/BCL2L14 with ab184925 at 1/250 dilution, followed by Goat anti-rabbit I Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab184925 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free (ab250782)

All lanes : Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925) at 1/2000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
Lane 3 : A549 (Human lung carcinoma) whole cell lysates
Lane 4 : Human fetal liver lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

This data was developed using ab184925, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free (ab250782)

Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925) at 1/5000 dilution + Human testis lysates at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

This data was developed using ab184925, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free (ab250782)

All lanes : Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925) at 1/2000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Mouse heart lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse spleen lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lane 7 : C6 (Rat glial tumor cells) whole cell lysates
Lane 8 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 10 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

This data was developed using ab184925, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free (ab250782)

This data was developed using ab184925, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Bcl G/BCL2L14 with ab184925 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary at 1/500 dilution. Cytoplasm staining on lymphocytes of Human tonsil is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. J Biol Chem. 2001 Jan 26;276(4):2780-5. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free (ab250782)

This data was developed using ab184925, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Bcl G/BCL2L14 with ab184925 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary at 1/500 dilution. Cytoplasmic staining on tumor cells of Human cervical squamous cell carcinoma is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free (ab250782)

This data was developed using ab184925, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Bcl G/BCL2L14 with ab184925 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary at 1/500 dilution. Cytoplasmic staining on mouse kidney tubules is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free (ab250782)

This data was developed using ab184925, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Bcl G/BCL2L14 with ab184925 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary at 1/500 dilution. Cytoplasmic staining on glandular epithelium of rat colon is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free (ab250782)

This data was developed using ab184925, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Bcl G/BCL2L14 with ab184925 at 1/130 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free (ab250782)

This data was developed using ab184925, the same antibody clone in a different buffer formulation.Bcl G/BCL2L14 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab184925 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184925 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: Rabbit monoclonal IgG (ab172730) instead of ab184925 in HeLa whole cell extract. Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-Bcl G/BCL2L14 antibody [EPR17666] - BSA and Azide free (ab250782)