Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2281(3)] to FGFR3 (phospho Y724)
  • Suitable for: Flow Cyt, Dot blot, WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)]
    See all FGFR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR2281(3)] to FGFR3 (phospho Y724)

  • Host species

    Rabbit

  • Specificity

    ab155960 only detects FGFR3 when phosphorylated at tyrosine 724.

  • Tested Applications & Species

    Application Species
    Dot
    Human
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human FGFR3 (phospho Y724). The exact sequence is proprietary.
    Database link: P22607

  • Positive control

    • WB: MCF7 lysate treated with pervanadate. ICC/IF: MCF7 cells treated with pervanadate. IP: MCF7 cells treated with pervanadate. Flow Cyt: MCF7 cells

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
    Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
    All lanes : Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960) at 1/20000 dilution (purified)

    Lane 1 : MCF7 cell lysate - untreated
    Lane 2 : MCF7 cell lysate - treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 88 kDa
    Observed band size: 96 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
    Flow Cytometry - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)

    Flow Cytometry analysis of MCF7 (human breast carcinoma) treated with 1 mM pervanadate for 30 minutes cells labeling FGFR3 (phospho Y724) with purified ab155960 at 1/50 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. Green shows untreated MCF7 (human breast carcinoma) cells. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Immunocytochemistry/ Immunofluorescence - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
    Immunocytochemistry/ Immunofluorescence - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)

    Immunocytochemistry/Immunofluorescence analysis of untreated, Per treated and Per + LP treated MCF7 cells labelling FGFR3 (phospho Y724) with ab155960 (left) and FGFR3 with ab137084 (right) both at a dilution of 1/200.

    Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    The image shows increased cytoplasmic staining after Pervanadate (1 mM, 30 min) treatment on MCF7 cells. The LP treatment decreased the cytoplasmic staining caused by Pervanadate.

    ab137084 was used as a Pan control for ab155960. The results showed cytoplasmic staining on untreated, Per treated and Per + LP treated MCF7 cells.

  • Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
    Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
    All lanes : Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960) at 1/10000 dilution (unpurified)

    Lane 1 : MCF7 whole cell lysate - treated with pervanadate
    Lane 2 : MCF7 whole cell lysate - untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 88 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
    Dot Blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)

    Dot blot analysis of human FGFR3 (pY724) phospho peptide (lane 1) and human FGFR3 non-phospho peptide (lane 2) labelling FGFR2 (phospho Y724) with ab155960 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
    Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)

    ab155960 (unpurified) at 1/15 immunoprecipitating FGFR3 (phospho Y724) in MCF7 cells treated with pervanadate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
    Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)

    ab155960 (purified) at 1/50 immunoprecipitating FGFR3 (phospho Y724) in MCF7 cells treated with pervanadate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
    Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)

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