Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGFR3 antibody [EPR2304(3)] - BSA and Azide free (ab187341)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2304(3)] to FGFR3 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-FGFR3 antibody [EPR2304(3)] - BSA and Azide free
    See all FGFR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR2304(3)] to FGFR3 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    We have tested this antibody in positive Mouse and Rat tissue lysates in WB and could not obtain a specific band for FGFR3. Please get in touch with our Scientific Support team if you wish to share any data regarding this antibody’s cross-reactivity in Mouse and Rat species.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab195871)

  • Positive control

    • WB: K562, HepG2 and HEK293 lysates.

  • General notes

    Ab187341 is the carrier-free version of ab133644. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab187341 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FGFR3 antibody [EPR2304(3)] - BSA and Azide free (ab187341)
    Western blot - Anti-FGFR3 antibody [EPR2304(3)] - BSA and Azide free (ab187341)
    All lanes : Anti-FGFR3 antibody [EPR2304(3)] (ab133644) at 1/2000 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 88 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-FGFR3 antibody [EPR2304(3)] - BSA and Azide free (ab187341)
    Western blot - Anti-FGFR3 antibody [EPR2304(3)] - BSA and Azide free (ab187341)
    All lanes : Anti-FGFR3 antibody [EPR2304(3)] (ab133644) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293 whole cell lysate
    Lane 2 : FGFR3 knockout HEK-293 whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 88 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab133644 observed at 125kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab133644 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in FGFR3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FGFR3 knockout samples were subjected to SDS-PAGE. Ab133644 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133644).

  • OI-RD Scanning - Anti-FGFR3 antibody [EPR2304(3)] - BSA and Azide free (ab187341)
    OI-RD Scanning - Anti-FGFR3 antibody [EPR2304(3)] - BSA and Azide free (ab187341)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133644).

  • Anti-FGFR3 antibody [EPR2304(3)] - BSA and Azide free (ab187341)
    Anti-FGFR3 antibody [EPR2304(3)] - BSA and Azide free (ab187341)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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