Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] - BSA and Azide free (ab271918)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2281(3)] to FGFR3 (phospho Y724) - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB, Dot blot, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] - BSA and Azide free
    See all FGFR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR2281(3)] to FGFR3 (phospho Y724) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WB, Dot blot, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7 lysate treated with pervanadate. ICC/IF: MCF7 cells treated with pervanadate. IP: MCF7 cells treated with pervanadate. Flow Cyt: MCF7 cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    ab271918 is the carrier-free version of ab155960. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-FGFR3 antibody [EPR2281(3)] - BSA and Azide free (ab271918)
    Flow Cytometry - Anti-FGFR3 antibody [EPR2281(3)] - BSA and Azide free (ab271918)

    Flow Cytometry analysis of MCF7 (human breast carcinoma) treated with 1 mM pervanadate for 30 minutes cells labeling FGFR3 (phospho Y724) with purified ab155960 at 1/50 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. Green shows untreated MCF7 (human breast carcinoma) cells. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155960).

  • Immunocytochemistry/ Immunofluorescence - Anti-FGFR3 antibody [EPR2281(3)] - BSA and Azide free (ab271918)
    Immunocytochemistry/ Immunofluorescence - Anti-FGFR3 antibody [EPR2281(3)] - BSA and Azide free (ab271918)

    Immunocytochemistry/Immunofluorescence analysis of untreated, Per treated and Per + LP treated MCF7 cells labelling FGFR3 (phospho Y724) with ab155960 (left) and FGFR3 with ab137084 (right) both at a dilution of 1/200.

    Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    The image shows increased cytoplasmic staining after Pervanadate (1 mM, 30 min) treatment on MCF7 cells. The LP treatment decreased the cytoplasmic staining caused by Pervanadate.

    ab137084 was used as a Pan control for ab155960. The results showed cytoplasmic staining on untreated, Per treated and Per + LP treated MCF7 cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155960).

  • Dot Blot - Anti-FGFR3 antibody [EPR2281(3)] - BSA and Azide free (ab271918)
    Dot Blot - Anti-FGFR3 antibody [EPR2281(3)] - BSA and Azide free (ab271918)

    Dot blot analysis of human FGFR3 (pY724) phospho peptide (lane 1) and human FGFR3 non-phospho peptide (lane 2) labelling FGFR2 (phospho Y724) with ab155960 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155960).

  • Immunoprecipitation - Anti-FGFR3 antibody [EPR2281(3)] - BSA and Azide free (ab271918)
    Immunoprecipitation - Anti-FGFR3 antibody [EPR2281(3)] - BSA and Azide free (ab271918)

    ab155960 (unpurified) at 1/15 immunoprecipitating FGFR3 (phospho Y724) in MCF7 cells treated with pervanadate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155960).

  • Immunoprecipitation - Anti-FGFR3 antibody [EPR2281(3)] - BSA and Azide free (ab271918)
    Immunoprecipitation - Anti-FGFR3 antibody [EPR2281(3)] - BSA and Azide free (ab271918)

    ab155960 (purified) at 1/50 immunoprecipitating FGFR3 (phospho Y724) in MCF7 cells treated with pervanadate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155960).

  • Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] - BSA and Azide free (ab271918)
    Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] - BSA and Azide free (ab271918)

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