Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGFR3 antibody [EPR2304(3)] (ab133644)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2304(3)] to FGFR3
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-FGFR3 antibody [EPR2304(3)]
    See all FGFR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR2304(3)] to FGFR3

  • Host species

    Rabbit

  • Specificity

    We have tested this antibody in positive Mouse and Rat tissue lysates in WB and could not obtain a specific band for FGFR3. Please get in touch with our Scientific Support team if you wish to share any data regarding this antibody’s cross-reactivity in Mouse and Rat species.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human FGFR3 aa 1-100 (extracellular). The exact sequence is proprietary.
    Database link: P22607
    (Peptide available as ab195871)

  • Positive control

    • WB: K562, HepG2 and HEK293 lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-FGFR3 antibody [EPR2304(3)] (ab133644)
    Western blot - Anti-FGFR3 antibody [EPR2304(3)] (ab133644)
    All lanes : Anti-FGFR3 antibody [EPR2304(3)] (ab133644) at 1/2000 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 88 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-FGFR3 antibody [EPR2304(3)] (ab133644)
    Western blot - Anti-FGFR3 antibody [EPR2304(3)] (ab133644)
    All lanes : Anti-FGFR3 antibody [EPR2304(3)] (ab133644) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293 whole cell lysate
    Lane 2 : FGFR3 knockout HEK-293 whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 88 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab133644 observed at 125kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab133644 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in FGFR3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FGFR3 knockout samples were subjected to SDS-PAGE. Ab133644 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • OI-RD Scanning - Anti-FGFR3 antibody [EPR2304(3)] (ab133644)
    OI-RD Scanning - Anti-FGFR3 antibody [EPR2304(3)] (ab133644)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FGFR3 antibody [EPR2304(3)] (ab133644)
    Anti-FGFR3 antibody [EPR2304(3)] (ab133644)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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