Anti-Fas antibody (ab82419)
Key features and details
- Rabbit polyclonal to Fas
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Fas antibody
See all Fas primary antibodies -
Description
Rabbit polyclonal to Fas -
Host species
Rabbit -
Specificity
Recognizes human, mouse and rat Fas. Detects a band of ~45kDa by Western blot. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide (Mouse) derived from the extracellular domain, conjugated to KLH
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Fas antibody (ab82419) Image courtesy of an anonymous AbreviewAll lanes : Anti-Fas antibody (ab82419) at 1/3000 dilution
Lane 1 : Mouse brain whole tissue lysate
Lane 2 : Mouse retina whole tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : ECL Rabbit IgG, HRP-linked whole Ab from donkey at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 5 minutesGel Running conditions:
Reduced Denaturing (4-12% BisTris Gel)
Blocking step:
Milk as blocking agent for 1 hour(s) and 0 minute(s).
Concentration: 5% - Temperature: 22°C
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Western blot - Anti-Fas antibody (ab82419) Image courtesy of an anonymous Abreview.All lanes : Anti-Fas antibody (ab82419) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : ECL Rabbit IgG, HRP-linked whole Ab from donkey at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 5 minutesGel Running Conditions:
Reduced Denaturing (4 -12% BisTris Gel)
Blocking step:
Milk as blocking agent for 1 hour(s) and 0 minute(s). Concentration: 5% - Temperature: 4°C
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Western blot - Anti-Fas antibody (ab82419)All lanes : Anti-Fas antibody (ab82419) at 1/1000 dilution
Lane 1 : Rat brain tissue extract
Lane 2 : Mouse brain tissue extract
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDa
