Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21088] to Fas
Suitable for: Flow Cyt, ICC/IF
Reacts with: Mouse

Product name

Anti-Fas antibody [EPR21088]
See all Fas primary antibodies
Description

Rabbit monoclonal [EPR21088] to Fas
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Mouse

ICC/IF
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: Primary mouse thymocytes. Flow Cyt: Primary mouse thymocytes.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21088
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Fas antibody [EPR21088] (ab216991)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized primary mouse thymocytes labeling Fas with ab216991 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on primary mouse thymocytes.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control: L-929 (mouse connective tissue fibroblast cell line) (PMID: 7521365).
Flow Cytometry - Anti-Fas antibody [EPR21088] (ab216991)

Flow cytometric analysis of primary mouse thymocytes labeling Fas with ab216991 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Total viable cells were gated for the image.
Flow Cytometry - Anti-Fas antibody [EPR21088] (ab216991)

Negative control: Flow cytometric analysis of L-929 (mouse connective tissue fibroblast cell line) cell line labeling Fas with ab216991 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: L-929 (PMID:12077239).
Anti-Fas antibody [EPR21088] (ab216991)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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