Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fas antibody [EPR21088] (ab216991)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21088] to Fas
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Fas antibody [EPR21088]
    See all Fas primary antibodies

  • Description

    Rabbit monoclonal [EPR21088] to Fas

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Primary mouse thymocytes. Flow Cyt: Primary mouse thymocytes.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Fas antibody [EPR21088] (ab216991)
    Immunocytochemistry/ Immunofluorescence - Anti-Fas antibody [EPR21088] (ab216991)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized primary mouse thymocytes labeling Fas with ab216991  at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on primary mouse thymocytes. 

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: L-929 (mouse connective tissue fibroblast cell line) (PMID: 7521365).

  • Flow Cytometry - Anti-Fas antibody [EPR21088] (ab216991)
    Flow Cytometry - Anti-Fas antibody [EPR21088] (ab216991)

    Flow cytometric analysis of primary mouse thymocytes labeling Fas with ab216991 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Total viable cells were gated for the image.

  • Flow Cytometry - Anti-Fas antibody [EPR21088] (ab216991)
    Flow Cytometry - Anti-Fas antibody [EPR21088] (ab216991)

    Negative control: Flow cytometric analysis of L-929 (mouse connective tissue fibroblast cell line) cell line labeling Fas with ab216991 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: L-929 (PMID:12077239).

  • Anti-Fas antibody [EPR21088] (ab216991)
    Anti-Fas antibody [EPR21088] (ab216991)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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