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Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12439] to GRASP65 - C-terminal
  • Suitable for: ICC/IF, Flow Cyt, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GRASP65 antibody [EPR12439] - C-terminal
    See all GRASP65 primary antibodies

  • Description

    Rabbit monoclonal [EPR12439] to GRASP65 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GRASP65 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BQQ3-1

  • Positive control

    • 293T, HeLa, HepG2 and MCF7 cell lysates; Human colon and uterus tissues; HepG2 and HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
    Immunocytochemistry/ Immunofluorescence - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

    Immunofluorescent analysis of HepG2 cells, labeling GRASP65 with ab174834 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Western blot - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
    Western blot - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
    All lanes : Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling GRASP65 with ab174834 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue, labeling GRASP65 with ab174834 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
    Flow Cytometry - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

    Flow cytometry analysis of permeabilized HeLa cells labeling GRASP65 with ab174834 at 1/10 dilution (red), or negative control rabbit IgG (green).

  • Immunoprecipitation - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
    Immunoprecipitation - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling GRASP65, immunoprecipitated using ab174834 at 1/10 dilution.

  • Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
    Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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