Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Bok antibody [BOK-R1-5-1] (ab233072)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BOK-R1-5-1] to Bok
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Bok antibody [BOK-R1-5-1]
See all Bok primary antibodies
Description

Rabbit monoclonal [BOK-R1-5-1] to Bok
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MEF, HepG2, MCF7 and C6 whole cell lysate; Human fetal liver, kidney and brain lysate; Mouse brain and liver lysate; Rat brain and spleen lysate. Flow Cytometry: HepG2 and MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

BOK-R1-5-1
Isotype

IgG
Research areas

Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

All lanes : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution

Lane 1 : Wild-type MEF (Mouse embryonic fibroblast cell line) cell lysate
Lane 2 : Bok knockout MEF cell lysate
Lane 3 : Wild-type HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : Bok knockout HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 23 kDa

Exposure time: 70 seconds

Blocking/diluting buffer and concentration: 5% NFDM/TBST.

The cell lysates were kindly provided by our collaborator Dr. Thomas Kaufmann, University of Bern, Switzerland.
Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

Lanes 1-3 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution
Lanes 4-9 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : Human fetal liver at 20 µg
Lane 3 : Human fetal kidney at 20 µg
Lane 4 : Mouse brain at 10 µg
Lane 5 : Mouse liver at 10 µg
Lane 6 : Rat brain at 10 µg
Lane 7 : Rat spleen at 10 µg
Lane 8 : C6 (rat glial tumor cell) whole cell lysate at 10 µg
Lane 9 : Human fetal brain at 10 µg

Secondary
Lanes 1-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 9 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 23 kDa

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: Lanes 1-3 and 8: 70 seconds; Lanes 4-5: 125 seconds; Lanes 6-7: 37 seconds; Lane 9: 3 minutes.

The specific band for BOK is observed at 22 kDa; the other bands on the blots are non-specific.
Flow Cytometry - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Anti-Bok antibody [BOK-R1-5-1] (ab233072)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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