Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15331] to Bok
Suitable for: WB, IP
Reacts with: Rat, Human

Product name

Anti-Bok antibody [EPR15331]
See all Bok primary antibodies
Description

Rabbit monoclonal [EPR15331] to Bok
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain, fetal kidney and ovary cancer lysate; HeLa and PC-3 cell lysates; Rat spleen lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15331
Isotype

IgG
Research areas

Western blot - Anti-Bok antibody [EPR15331] (ab186745)

All lanes : Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human ovary cancer lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 23 kDa
Observed band size: 23 kDa
Western blot - Anti-Bok antibody [EPR15331] (ab186745)

All lanes : Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : PC-3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 23 kDa
Observed band size: 23 kDa
Western blot - Anti-Bok antibody [EPR15331] (ab186745)

Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution + Rat spleen lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 23 kDa
Observed band size: 23 kDa
Immunoprecipitation - Anti-Bok antibody [EPR15331] (ab186745)

Western blot analysis of Bok in PC-3 cell lysate immunoprecipitated with ab186745 at 1/50 dilution (Lane1). Lane 2: PBS instead of PC-3 cell lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Anti-Bok antibody [EPR15331] (ab186745)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com