Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10959(B)] to MPG/AAG - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free
    See all MPG/AAG primary antibodies

  • Description

    Rabbit monoclonal [EPR10959(B)] to MPG/AAG - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249186 is the carrier-free version of ab155092 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249186 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as MPG

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    Western blot - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)

    This data was developed using ab155092, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: MPG/AAG knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: HEK293 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab155092 observed at 35 kDa. Red - loading control, ab8245.

    ab155092 was shown to specifically react with MPG/AAG when MPG/AAG knockout samples were used. Wild-type and MPG/AAG knockout samples were subjected to SDS-PAGE. ab155092 and ab8245 (loading control to GAPDH) were diluted at 1/10,000 and 1/10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)

    This data was developed using ab155092, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155092 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    Western blot - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    All lanes : Anti-MPG/AAG antibody [EPR10959(B)] (ab155092) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 33 kDa



    This data was developed using ab155092, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    Flow Cytometry - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)

    This data was developed using ab155092, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MPG/AAG with unpurified ab155092 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)

    This data was developed using ab155092, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MPG/AAG with ab155092 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)

    This data was developed using ab155092, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tesis tissue labeling MPG/AAG with ab155092 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    This data was developed using ab155092, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155092 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    This data was developed using ab155092, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155092 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    This data was developed using ab155092, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155092 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)
    Anti-MPG/AAG antibody [EPR10959(B)] - BSA and Azide free (ab249186)

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