Anti-Cytokeratin 18 antibody (ab137860)
Key features and details
- Rabbit polyclonal to Cytokeratin 18
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 18 antibody
See all Cytokeratin 18 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 18 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 164-425 of Human Cytokeratin 18 (NP_000215, NP_954657).
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Positive control
- Recombinant Human Cytokeratin 18 protein (ab73638) can be used as a positive control in WB. HeLa cells and whole cell lysate, A431, H1299, HepG2, Molt4, Raji whole cell lysates, H661 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling Cytokeratin 18 with ab137860 at 1/200. Lower panel shows mergence with DNA probe.
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Anti-Cytokeratin 18 antibody (ab137860) at 1/10000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin embedded H661 xenograft tissue labelling Cytokeratin 18 with ab137860 at 1/500.