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Anti-TLE 1 antibody [TLE1/2051] - BSA and Azide free (ab237914)

Key features and details

  • Mouse monoclonal [TLE1/2051] to TLE 1 - BSA and Azide free
  • Suitable for: Protein Array, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-TLE 1 antibody [TLE1/2051] - BSA and Azide free
    See all TLE 1 primary antibodies

  • Description

    Mouse monoclonal [TLE1/2051] to TLE 1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Protein Array, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant fragment within Human TLE 1 aa 175-338. The exact sequence is proprietary.
    Database link: Q04724

  • Positive control

    • IHC-P: Human endometrial carcinoma tissue. WB: MCF7, HepG2, SH-SY5Y and HeLa whole cell lysate.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-TLE 1 antibody [TLE1/2051] - BSA and Azide free (ab237914)
    Western blot - Anti-TLE 1 antibody [TLE1/2051] - BSA and Azide free (ab237914)
    All lanes : Anti-TLE 1 antibody [TLE1/2051] (ab238063) at 0.5 µg/ml

    Lane 1 : Wild-type MCF7 cell lysate
    Lane 2 : TLE1 knockout MCF7 cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab238063).

    This product was validated with an edited cell line that does not express the epitope recognised by this antibody. Testing with other antibodies has shown that the edited cell line produces a truncated protein.

    Lanes 1 - 4: Merged signal (red and green). Green - ab238063 observed at 83 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

    ab238063 was shown to react with TLE 1 in wild-type MCF7 cells in Western blot with loss of signal observed in TLE1 knockout sample. Wild-type MCF7 and TLE1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab238063 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at 0.5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Protein Array - Anti-TLE 1 antibody [TLE1/2051] - BSA and Azide free (ab237914)
    Protein Array - Anti-TLE 1 antibody [TLE1/2051] - BSA and Azide free (ab237914)
    This data was produced with ab238063, the same antibody in a different formulation with BSA and Azide.
    ab238063 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [TLE1/2051] (ab237914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [TLE1/2051] (ab237914)

    Formalin-fixed, paraffin-embedded human endometrial carcinoma tissue stained for TLE 1 using ab237914 at 2 μg/mL in immunohistochemical analysis.

  • Western blot - Anti-TLE 1 antibody [TLE1/2051] (ab237914)
    Western blot - Anti-TLE 1 antibody [TLE1/2051] (ab237914)
    Anti-TLE 1 antibody [TLE1/2051] - BSA and Azide free (ab237914) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Predicted band size: 83 kDa

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