Anti-ZCCHC4 antibody (ab209901)
Key features and details
- Rabbit polyclonal to ZCCHC4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZCCHC4 antibody
See all ZCCHC4 primary antibodies -
Description
Rabbit polyclonal to ZCCHC4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human ZCCHC4 aa 50-200.
Database link: Q9H5U6 -
Positive control
- IHC-P: Human testis, uterine cervix, lung and kidney tissue; WB: U-251MG cell line lysate; Human plasma, liver and tonsil tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC4 antibody (ab209901)
Immunohistochemical analysis of human testis tissue labeling ZCCHC4 in the cytoplasm and nucleus of cells in seminiferous ducts with ab209901 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-ZCCHC4 antibody (ab209901)All lanes : Anti-ZCCHC4 antibody (ab209901) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC4 antibody (ab209901)Immunohistochemical analysis of human uterine cervix tissue labeling ZCCHC4 in the cytoplasm and nucleus of squamous epithelial cells with ab209901 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC4 antibody (ab209901)Immunohistochemical analysis of human lung tissue labeling ZCCHC4 in the cytoplasm of macrophages with ab209901 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC4 antibody (ab209901)Immunohistochemical analysis of human kidney tissue labeling ZCCHC4 in the cytoplasm of cells in tubules with ab209901 at a 1/20 diution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
