Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ZCCHC4 antibody [EPR20492] (ab211325)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20492] to ZCCHC4
Suitable for: WB, Flow Cyt, IP
Knockout validated
Reacts with: Human

Product name

Anti-ZCCHC4 antibody [EPR20492]
See all ZCCHC4 primary antibodies
Description

Rabbit monoclonal [EPR20492] to ZCCHC4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild type HeLa whole cell lysate. Flow Cyt: HeLa and HepG2 cells. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20492
Isotype

IgG
Research areas

Western blot - Anti-ZCCHC4 antibody [EPR20492] (ab211325)

All lanes : Anti-ZCCHC4 antibody [EPR20492] (ab211325) at 1/1000 dilution

Lane 1 : Wild type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : ZCCHC4 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 59 kDa
Observed band size: 59 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

This antibody works best on a semi-dry nitrocellulose membrane transfer system.

These lysates were kindly provided by Prof Chuan He; University of Chicago.
Flow Cytometry - Anti-ZCCHC4 antibody [EPR20492] (ab211325)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ZCCHC4 with ab211325 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Anti-ZCCHC4 antibody [EPR20492] (ab211325)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling ZCCHC4 with ab211325 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-ZCCHC4 antibody [EPR20492] (ab211325)

ZCCHC4 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab211325 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab211325 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: HeLa whole cell lysate 10 µg (Input).

Lane 2: ab211325 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211325 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.

This antibody works best on a semi-dry nitrocellulose membrane transfer system.
Anti-ZCCHC4 antibody [EPR20492] (ab211325)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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