Anti-c-Myb antibody (ab226251)
Key features and details
- Rabbit polyclonal to c-Myb
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Myb antibody
See all c-Myb primary antibodies -
Description
Rabbit polyclonal to c-Myb -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human c-Myb aa 525-575. The exact sequence is proprietary. (NP_005366.2).
Database link: P10242 -
Positive control
- WB: MOLT-4 and Jurkat whole cell lysate (ab7899). IP: MOLT-4 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226251 was affinity purified using an epitope specific to c-Myb immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-Myb antibody (ab226251)All lanes : Anti-c-Myb antibody (ab226251) at 0.1 µg/ml
Lane 1 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 50 µg
Lane 2 : MOLT-4 whole cell lysate at 15 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-c-Myb antibody (ab226251)c-Myb was immunoprecipitated from MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226251 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226251 at 0.4 µg/ml.
Lane 1: ab226251 IP in MOLT-4 whole cell lysate.
Lane 2: Control IgG IP in MOLT-4 whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
