Anti-c-Myb antibody [ANA236B] (ab169111)
![Anti-c-Myb antibody [ANA236B] (ab169111)](https://www.abcam.com/ps/products/169/ab169111/Images/ab169111-297399-anti-c-myb-antibody-ana236b-western-blot.jpg "Anti-c-Myb antibody [ANA236B] (ab169111)")
Key features and details
- Rat monoclonal [ANA236B] to c-Myb
- Suitable for: WB, IHC-P, ICC
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-c-Myb antibody [ANA236B]
See all c-Myb primary antibodies -
Description
Rat monoclonal [ANA236B] to c-Myb -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment (His-tag) corresponding to Human c-Myb aa 402-640.
Sequence:FLNTSSNHE NSDLEMPSLT STPLIGHKLT VTTPFHRDQT VKTQKENTVF RTPAIKRSIL ESSPRTPTPF KHALAAQEIK YGPLKMLPQT PSHLVEDLQD VIKQESDESG IVAEFQENGP PLLKKIKQEV ESPTDKSGNF FCSHHWEGDS LNTQLFTQTS PVADAPNILT SSVLMAPASE DEDNVLKAFT VPKNRSLASP LQPCSSTWEP ASCGKMEEQM TSSSQARKYV NAFSARTLVM
Database link: P10242 -
Positive control
- Recombinant Human c-Myb protein (ab114406) can be used as a positive control in WB. Hek-V5-c-Myb transfected cells; KARPAS299, Jurkat, MT2 and SUP-T1 cell lysates; Human thymus, bone marrow and lymphoblastic lymphoma tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
ANA236B -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-c-Myb antibody [ANA236B] (ab169111)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: c-Myb knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab169111 observed at 75 kDa. Red - loading control, ab181602, observed at 37 kDa.
Ab169111 was shown to specifically react with c-Myb, in wild type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected SDS-PAGE. Ab169111 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/50 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) preabsorbed and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody [ANA236B] (ab169111)](https://www.abcam.com/ps/products/169/ab169111/Images/ab169111-171138-anti-c-myb-antibody-ana236b-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody [ANA236B] (ab169111)Immunohistochemical analysis of paraffin-embedded Human lymphoblastic lymphoma tissue labeling c-Myb with ab169111 at 1/80 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody [ANA236B] (ab169111)](https://www.abcam.com/ps/products/169/ab169111/Images/ab169111-171137-anti-c-myb-antibody-ana236b-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody [ANA236B] (ab169111)Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling c-Myb with ab169111 at 1/80 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody [ANA236B] (ab169111)](https://www.abcam.com/ps/products/169/ab169111/Images/ab169111-170985-anti-c-myb-antibody-ana236b-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody [ANA236B] (ab169111)Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling c-Myb with ab169111 at 1/80 dilution.
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![Western blot - Anti-c-Myb antibody [ANA236B] (ab169111)](https://www.abcam.com/ps/products/169/ab169111/Images/ab169111-170983-anti-c-myb-antibody-ana236b-western-blot.jpg)
Western blot - Anti-c-Myb antibody [ANA236B] (ab169111)All lanes : Anti-c-Myb antibody [ANA236B] (ab169111) at 1/50 dilution
Lane 1 : DAUDI cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Raji cell lysate
Lane 4 : KARPAS299 cell lysate
Lane 5 : HUT78 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : MT2 cell lysate
Lane 8 : SUP-T1 cell lysate
Lane 9 : YT cell lysate
Lysates/proteins at 150 µg per lane.
Predicted band size: 72 kDa
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![Immunocytochemistry - Anti-c-Myb antibody [ANA236B] (ab169111)](https://www.abcam.com/ps/products/169/ab169111/Images/ab169111-170980-anti-c-myb-antibody-ana236b-immunocytochemistry.jpg)
Immunocytochemistry - Anti-c-Myb antibody [ANA236B] (ab169111)Immunocytochemical analysis of Hek-V5-c-Myb transfected cells cytospins (right image) labeling c-Myb using ab169111. ab169111 does not detect other Myb proteins in Hek-V5-a-Myb transfected cells cytospins (left image) or in Hek-V5-b-Myb transfected cells cytospins (middle image).
