Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RUNX3 antibody [R3-5G4] (ab40278)

Key features and details

  • Mouse monoclonal [R3-5G4] to RUNX3
  • Suitable for: Flow Cyt, WB, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-RUNX3 antibody [R3-5G4]
    See all RUNX3 primary antibodies

  • Description

    Mouse monoclonal [R3-5G4] to RUNX3

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human RUNX3 aa 150 to the C-terminus (C terminal).

  • Positive control

    • ab40278 gave a positive result in the following whole cell lysates: Jurkat (Human T cell lymphoblast-like cell line) Raji (Human Burkitt's lymphoma cell line) SW480 (Human colon adenocarcinoma cell line)

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...

  • Purity

    IgG fraction

  • Clonality

    Monoclonal

  • Clone number

    R3-5G4

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-RUNX3 antibody [R3-5G4] (ab40278)
    Western blot - Anti-RUNX3 antibody [R3-5G4] (ab40278)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: RUNX3 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab40278 observed at 44 kDa. Red - loading control, ab176560, observed at 50 kDa.

    ab40278 was shown to specifically react with RUNX3 in wild-type HAP1 cells as signal was lost in RUNX3 knockout cells. Wild-type and RUNX3 knockout samples were subjected to SDS-PAGE. ab40278 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX3 antibody [R3-5G4] (ab40278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX3 antibody [R3-5G4] (ab40278)
    IHC image of RUNX3 staining in human lymphoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40278, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Flow Cytometry - Anti-RUNX3 antibody [R3-5G4] (ab40278)
    Flow Cytometry - Anti-RUNX3 antibody [R3-5G4] (ab40278)
    Overlay histogram showing HEK293 cells stained with ab40278 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40278, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-RUNX3 antibody [R3-5G4] (ab40278)
    Western blot - Anti-RUNX3 antibody [R3-5G4] (ab40278)
    All lanes : Anti-RUNX3 antibody [R3-5G4] (ab40278) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : SW480 whole cell lysate (ab3957)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Rabbit Anti-Mouse IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Additional bands at: 46 kDa (possible isoform)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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