Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PDX1 antibody [EPR22002] (ab219207)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22002] to PDX1
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-PDX1 antibody [EPR22002]
See all PDX1 primary antibodies
Description

Rabbit monoclonal [EPR22002] to PDX1
Host species

Rabbit
Specificity

Human and rat species are recommended based on IHC result. This antibody is unsuitable to be used in western blot on human and rat samples.

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IHC-P	Mouse Rat Human
IP	Mouse
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse Beta-TC-6 whole cell lysate. IHC-P: Mouse, rat and human pancreas tissues. ICC/IF: Beta-TC-6 cells. IP: Beta-TC-6 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22002
Isotype

IgG
Research areas

Western blot - Anti-PDX1 antibody [EPR22002] (ab219207)

All lanes : Anti-PDX1 antibody at 1/1000 dilution

Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 31 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Lane 1: 60 seconds

Lane 2: 7 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (ab219207)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining in mouse pancreatic islet (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

Performed on a Leica Biosystems BOND® RX instrument.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-PDX1 antibody [EPR22002] (ab219207)

All lanes : Anti-PDX1 antibody [EPR22002] (ab219207) at 1/1000 dilution

Lane 1 : Beta-TC-6 (mouse pancreas insulinoma beta cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 31 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?

Exposure time: 92 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID: 12488243)

Negative control: NIH/3T3 (PMID: 25271154)
Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [EPR22002] (ab219207)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling PDX1 with ab219207 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining in Beta-TC-6 cells.

Negative control: NIH/3T3 (PMID: 25271154).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (ab219207)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining in rat pancreatic islet (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

Performed on a Leica Biosystems BOND® RX instrument.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (ab219207)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining on human pancreatic islet and pancreatic acini (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

Performed on a Leica Biosystems BOND® RX instrument.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-PDX1 antibody [EPR22002] (ab219207)

PFX1 was immunoprecipitated from 0.35 mg of Beta-TC-6 (mouse pancreas insulinoma beta cell line) whole cell lysate with ab219207 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219207 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: Beta-TC-6 whole cell lysate 10 µg (Input).
Lane 2: ab219207 IP in Beta-TC-6 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219207 in Beta-TC-6 whole cell lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 3 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID: 12488243).
Anti-PDX1 antibody [EPR22002] (ab219207)

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